Britney Spears told the men at her Las Vegas concert to "suck my f***ing toe".
The 33-year-old singer was performing during her 'Piece of Me' residency when she made the bizarre comment, after first praising the female members of her audience.
She said: "Look how beautiful the girls are, oh my God. Men, frankly, can suck my f***ing toe. But girls, I love you!"
Meanwhile, Britney - who has sons Sean Preston, nine and Jayden James, eight, with former husband Kevin Federline, is currently single since splitting up with producer Charlie Ebersol earlier this year.
While the pair are believed to still be friends, it has been claimed that they broke up because he didn't want to get married.
A source said: "Britney had been pressuring Charlie for the last several months about getting married. She is at a point where she wants to have another child, and very much wants to be married again. Charlie had been very honest about not being ready to get married."
Another added: "Britney has a lot of issues, and it's a lot for a young single bachelor to take on. The fact that Britney is still under a conservatorship is obviously very telling.
"She can't manage her own affairs. Charlie didn't want to become a father figure in her life. She was demanding it and made him feel trapped."
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