The 22-year-old singer famously wore a purity ring - which symbolised a promise not to have sex before marriage - as a member of the Jonas Brothers, which he formed with his siblings when he was 12 but after removing his jewellery in September, the hunky star has admitted he is "an adult in all ways".
He said: "Am I still a virgin? No. I am an adult in all ways and I am comfortable with who I am."
However, Nick - who is dating model Olivia Culpo - insists he doesn't "regret" wearing the ring because it was an important part of his "belief system" at the time.
He told talk show host Wendy Williams: "I don't regret [wearing a purity ring]. I think that it was an important part of my childhood.
"As I grew up, I sort of figured out what was important to me, my own belief system, which we all grow up, we all live life and find out what's important to us. I am now comfortable with who I am and what I believe in."
The 'Jealous' hitmaker has been in a relationship with former Miss Universe Olivia for 18 months and is very happy.
He said: "We're happy and we're enjoying the ride."
When Jonas Brothers split last year, Joe Jonas, 25, admitted he had lost his virginity when he was 20 years old. Their other brother and bandmate, 27-year-old Kevin married wife Danielle in 2009 and they have nine-month-old daughter Alena together.